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Ampli1 Library Preparation

Ampli1 Library Preparation

Library preparation kits tailored to your single cell needs

Ampli1™ LowPass Kit and Ampli1™ OncoSeek Panel enable the preparation of next-generation sequencing (NGS) libraries starting from the Ampli1 WGA product to detect genome-wide Copy Number Aberrations and hotspot mutations in the same single cell.

This workflow takes advantage of the genome-wide coverage and unbiased representation of alleles at genomic loci of Ampli1™ WGA kit.

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Ampli1 LowPass Kit

Ampli1 LowPass Kit generates multiplexed, sequencing-ready libraries in a streamlined single-day protocol, for detection of chromosomal aneuploidies and copy number aberrations (CNAs) by low-pass whole genome sequencing.

The Ampli1 LowPass Kit works exclusively with Ampli1 WGA product.

Ampli1 LowPass Kit is compatible with Ion Torrent systems (Ion PGM™ and Ion S5™) and with all main Illumina Sequencers ( MiSeq,  NovaSeqTM, NextSeq 500/550, NextSeq 1000/2000).

Product is for research use only (RUO) – not for use in diagnostic procedures.

Specific and quantitative detection of copy number aberrations (CNAs) in single CTC

Low-pass Whole Genome Profile (chr 1-22) of single CTC (lower panel) and WBC (upper panel) obtained with Ampli1 WGA kit and Ampli1 LowPass kit. Each cell was isolated with DEPArray™ Technology from a patient with Lung Adenocarcinoma. Ploidy values are indicated in the y-axis. CNAs in the CTC are indicated in red and blue.

Ampli1 OncoSeek Panel

Ampli1 OncoSeek Panel for Illumina® is the first and only panel specifically designed for single-cell WGA product analysis. It enables the simultaneous detection of single nucleotide variants (SNVs), insertions/deletions (indels) and copy-number amplifications (CNAs) in a panel of 60 clinically relevant oncology-related genes.

It requires only 10 ng of DNA and works exclusively with Ampli1 WGA product. By leveraging the deterministic nature of Ampli1 WGA, Ampli1 OncoSeek achieves a very low Allelic Dropout Rate (ADO=12.7% ± 4.2%).

Ampli1 OncoSeek Panel is compatible with all Illumina® platforms using non-patterned flow cells (MiSeq, MiniSeq, NextSeq 500/550).

The Panel is currently not available in North America.

Product is for research use only (RUO) – not for use in diagnostic procedures.

Ampli1 OncoSeek Panel targets 60 oncology-related genes
ABL1
5
CDK6
10
FBXW7
4
HRAS
2
MLH1
1
PTPN11
2
AKT1
2
CDKN2A
2
FGFR1
12
IDH1
1
MPL
1
RB1
7
ALK
13
CSF1R
2
FGFR2
13
IDH2
2
MSH6
1
RET
5
APC
15
CTNNB1
1
FGFR3
7
JAK2
1
MYC
9
SMAD4
9
AR
10
DDR2
1
FGFR4
9
JAK3
3
MYCN
9
SMARCB1
4
ATM
8
DNMT3A
1
FLT3
4
KDR
5
NOTCH1
3
SMO
5
BRAF
12
EGFR
19
GNA11
2
KIT
22
NRAS
3
SRC
1
CCND1
8
ERBB2
14
GNAQ
2
KRAS
11
PDGFRA
13
TP53
19
CDH1
2
ERBB4
4
GNAS
2
MAP2K1
4
PIK3CA
17
TSC1
1
CDK4
10
EZH2
1
HNF1A
4
MET
15
PTEN
8
VHL
2

Genes with contiguous, overlapping coverage;
Genes with amplicons for CNA, SNVs and InDel calling;
Genes with amplicons for CNA calling only.

The table reports the gene detected by the Panel and the number of amplicons per genes.

CODE
DESCRIPTION
 
84000031 / USA
KI0053 / EUROPE
Ampli1 LowPass Kit ION TORRENT Set A
 
84000032 / USA
KI0052 / EUROPE
Ampli1 LowPass Kit ION TORRENT Set B
 
84000065 / USA
KI0041 / EUROPE
Ampli1 LowPass Kit for Illumina - Set A
To be ordered / used together with the code 84000157 / KI0112
84000066 / USA
KI0040 / EUROPE
Ampli1 LowPass Kit for Illumina - Set B
To be ordered / used together with the code 84000157 / KI0112
84000157 / USA
KI0112 / EUROPE
Ampli1 LowPass Kit for Illumina - PEG NaCl
 
NA / USA
KI0116 / EUROPE
Ampli1 OncoSeek Panel for Illumina - Pre-PCR
To be ordered/ used together with codes KI0117, KI0118
NA / USA
KI0117 / EUROPE
Ampli1 OncoSeek Panel for Illumina - Post-PCR
To be ordered/ used together with codes KI0116, KI0118
NA / USA
KI0118 / EUROPE
Ampli1 OncoSeek Panel for Illumina - PEG NaCl
To be ordered/ used together with codes KI0116, KI0117

Products Resources

Visit our product resources page to access Instructions for Use and Material Safety Data Sheets (MSDSs) for all our products.

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Publications

Discover recently published articles featuring research enabled by our CELLSEARCH, DEPArray and Ampli1 technologies

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