The DEPArray Kits streamline and optimize the analysis of FFPE samples by providing DEPArray users a fast and easy workflow, for a precise genetic analysis on pure cancer and stromal cell populations.
The workflow includes:
The DEPArray FFPE SamplePrep Kit is intended for the dissociation of FFPE human tissue sections into a cell suspension and the subsequent immunofluorescence staining by anti-keratin and anti-vimentin antibodies.
The kit allows the detection, identification and isolation of vimentin-positive stromal cells and keratin-positive putative cancer cells with DEPArray system.
This product contains reagents that are subjected to specific Limitations of Use*.
Formalin-fixation causes fragmentation and chemical modifications in DNA, posing significant challenges for molecular assays, such as downstream NGS applications. The DEPArray FFPE QC Kit has been developed to assess the DNA integrity of cell suspensions obtained from formalin-fixed paraffin-embedded (FFPE) samples of human origin prior to DEPArray experiments and NGS library preparation.
DNA quality assessment is performed by a qPCR-based assay using two different primer pairs that produce amplicons of 54 bp and 132 bp. The quality of DNA is inferred by the difference in quantification cycles (Cq) obtained with the long amplicon assay and the short amplicon assay for a given sample.
The QC score, coupled with ploidy information, allows to predict the outcome of NGS library preparation, providing a useful tool to infer the minimum recommended number of cells to obtain a given NGS performance level.
DEPArray LysePrep Kit has been optimized for the lysis of up to 1500 fresh or fixed cells in order to release genomic DNA. The cells lysate obtained can be used directly for subsequent DNA analysis (Targeted PCR, NGS library preparation for Ion Torrent and Illumina platforms).
The DEPArray OncoSeek Panel enables the preparation of high-quality targeted NGS libraries from FFPE cell populations isolated with the DEPArray technology or from fresh tissue. It allows the simultaneous detection of SNVs, indels and CNAs from 63 oncology relevant genes starting from very low amount of input DNA, such as 60 fresh/unfixed cells corresponding to about 400pg DNA and around 100-300 FFPE cells depending on quality according to DEPArray FFPE QC Kit results.
The kit utilizes Illumina-compatible adapter sequences and has been validated on Illumina platforms.
The DEPArray kits are for Research Use Only. Not for use in diagnostic procedures.
*Limited use label license
Secondary Ab-Alexa Fluor®488 (AF488), Secondary Ab-Alexa Fluor®647 (AF647) are subject to an intellectual property license from Life Technologies Corporation. Accordingly, the user should take into account the following field of use limitations.
USE IN RESEARCH FIELD
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or email@example.com
USE IN CONTRACT RESEARCH SERVICE FIELD
This product is provided under an intellectual property license from Life Technologies Corporation that authorizes the use of such intellectual property by an end-user organization that provides support to the pharmaceutical, biotechnology, and/or medical device industries or to academic researchers in the form of research services outsourced on a contract basis (“Research Entity”) in connection with the purchased product solely for the purpose of performing research or development services (including under contract or as fee for service test) performed by a Research Entity to generate results for a Research Entity customer’s internal research and development activities. The transfer of this product is conditioned on the buyer not using this product for therapeutic or prophylactic purposes, for manufacture of another product, or any other purpose not expressly authorized. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or firstname.lastname@example.org.