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 Ampli1™ OncoSeek Panel for Illumina User Manual

 Ampli1™ OncoSeek Panel Flyer

 

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Ampli1™ OncoSeek Panel

 

The Ampli1™ OncoSeek Panel for Illumina® enables the preparation of high-quality targeted NGS libraries starting from whole genome amplified DNA obtained with  Ampli1 WGA Kit.

Ampli1_OncoSeek_WF

 

It allows the simultaneous detection of single nucleotide variants (SNVs), insertions/deletions (indels) and copy-number amplifications (CNAs) in a panel of 60 clinically relevant oncology-related genes.

Ampli1 OncoSeek_genes

 

The Ampli1™ OncoSeek Panel works exclusively with Ampli1™ WGA product. The complete workflow takes advantage of the isolation of 100% pure cells with the DEPArray™ system and balanced whole genome amplification using the Ampli1™ WGA kit. 

This kit contains reagents for a rapid preparation of up to 96 barcoded libraries compatible with Illumina ® platforms.

Main Features

  • First and only panel designed for single cell analysis
  • Coverage of 60 oncology-relevant genes including more than 2500 mutation hotspots and CNAs for a subset of 19 genes
  • Very Low Allelic Dropout Rate (ADO=12.7% ± 4.2%)
  • Automated bioinformatic analysis available on the MSBiosuite platform - Ampli1 OncoSeek pipeline

References

Maturo et al, Cancer Genomics 2019, "Development and Validation of Target Next-Generation Sequencing Panel for detection of somatic mutations and focal Copy Number Amplifications (CNAs) in single Circulating Tumor Cells (CTCs)"

Forcato et al, EACR-ESMO Liquid Biopsies 2019, "Comprehensive molecular profiling of single Circulating Tumor Cells (CTCs) reveals intra-patient heterogeneity"

Tononi et al, AACR 2019, "Validation of a targeted sequencing workflow for sequence variants and focal Copy Number Alterations (CNAs) in single Circulating Tumor Cells (CTCs)"

To download the posters please go to our Posters page.

To download Material Safety Data Sheets (MSDS), please click here.

The Ampli1™OncoSeek Panel is for Research Use Only. Not for use in diagnostic procedures. 
©2020 Menarini Silicon Biosystems. All rights reserved. This site is published by Menarini Silicon Biosystems which is solely responsible for its contents.The products/services discussed are for research use only and not for use in diagnostic procedures.