Ampli1™ workflow: 

Single Cell DNA analysis

Single-cell analysis allows the study of cell-to-cell variation within a cell population and  is a prerequisite for in-depth understanding of tumor heterogeneity and to study diseases and drug development.

Successful analysis of single cell genome starts with an appropriate Whole Genome Amplification (WGA) step and ends with an optimized sequencing process and bioinformatic analysis matching the characteristics of the adopted WGA.

Ampli1 kits provides a complete range of products covering the entire workflow and optimized for different kinds of genetic analysis on the amplified DNA.

• Ampli1™ WGA is the best performing WGA in terms of low allelic drop-out rate (ADO) and reproducibility of amplification bias for the analysis of true single cell, thanks to its totally controlled priming and cycling of the PCR reaction. Ampli1 QC Kit provides a useful quality control check of the WGA procedure to establish DNA integrity before downstream NGS analysis.
• Ampli1 LowPass Kit and Ampli1 OncoSeek Panel enable the preparation of next-generation sequencing (NGS) library starting from Ampli1 WGA product to detect genome-wide Copy Number Aberrations and hotspot mutations in the same single cell.
• MSBiosuite completes our  sample to result solution by automating the processing of sequencing data and providing highly confident variant calling and generation of copy number aberration profiles.  

To download Material Safety Data Sheets (MSDS), please click here.

The Ampli1™ WGA Kit, Ampli1 QC Kit, Ampli1 ReAmp/ds Kit, Ampli1 LowPass Kit, Ampli1 OncoSeek Panel and MSBiosuite are for Research Use Only. Not for use in diagnostic procedures. Ampli1™ WGA Kits are protected by U.S. patent 6,673,541 and corresponding patent claims outside the United States.