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DEPArray Kits

Streamline FFPE Samples Analysis

The DEPArray™ Kits streamline and optimize the analysis of FFPE samples by providing DEPArray™ users a fast and easy workflow, for a precise genetic analysis on pure cancer and stromal cell populations. The workflow includes a DNA quality control assay for FFPE samples - DEPArray™ FFPE QC Kit - a cell lysis kit downstream of DEPArray™ sorting - DEPArray™ LysePrep Kit - and an oncology panel library preparation kit for Next Generation Sequencing (NGS) – DEPArray™ OncoSeek Panel.

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DEPArray FFPE QC Kit

Formalin-fixation causes fragmentation and chemical modifications in DNA, posing significant challenges for molecular assays, such as downstream NGS applications. The DEPArray™ FFPE QC Kit has been developed to assess the DNA integrity of cell suspensions obtained from formalin-fixed paraffin-embedded (FFPE) samples of human origin prior to DEPArray™ experiments and NGS library preparation.Scatola_FFPE QC Kit.jpg

DNA quality assessment is performed by a qPCR-based assay using two different primer pairs that produce amplicons of 54 bp and 132 bp. The quality of DNA is inferred by the ratio between the quantification using the long amplicon and the short amplicon (QC score). The QC score, coupled with ploidy information, allows to predict the outcome of NGS library preparation, providing a useful tool to infer the minimum recommended number of cells to obtain a given NGS performance level.

 

DEPArray LysePrep Kit

DEPArray™ LysePrep Kit has been optimized for the lysis of up to 1500 fresh or fixed cells in order to release genomic DNA. The cells lysate obtained can be used directly for subsequent DNA analysis (Targeted PCR, NGS library preparation for Ion Torrent and Illumina platforms).
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Sample types

  • Fresh/live cells
  • Fixed with paraformaldehyde (1%-2% PFA, 10’-20’ at RT)
  • Cells isolated from blood samples collected in CellSave/Streck tubes
  • Inside Stain (Inside Fix/Inside Perm) from Miltenyi
  • Biotec GmbH
  • Cell suspension obtained from FFPE tissue specimens

Main Features

  • Simple one-step cell lysis procedure
  • There are no sample transfers, columns or washes, which can lead to loss and⁄or dilution of the precious sample
  • The entire lysate can be used in downstream reactions ensuring maximum sensitivity and accuracy in your experiments

DEPArray OncoSeek Panel

The DEPArray™ OncoSeek Panel enables the preparation of high-quality targeted NGS libraries from FFPE cell populations isolated with the DEPArray technology or from fresh tissue. It allows the simultaneous detection of SNVs, indels and CNAs from 63 oncology relevant genes starting from very low amount of input DNA, such as 60 fresh/unfixed cells corresponding to about 400pg DNA and around 100-300 FFPE cells depending on quality according to DEPArray FFPE QC Kit results.

The kit utilizes Illumina-compatible adapter sequences and has been validated on Illumina platforms. Scatola_DEPArray OncoSeek Panel_Warranty Seal.jpg

 

Main Features

  • Input as low as 60 fresh cells (400pg DNA). Around 100-300 FFPE cells (660-1980 pg).
  • Single Tube, 4 hours workflow
  • Coverage of 63 oncology relevant genes
  • Detection of SNPs, indels and CNAs
  • Leverages the high fidelity of the Illumina Platform
  • Enables multiplexing of up to 96 unique libraries

Request Information

 

To review publications and resource references, please visit our Publications page. For more information about FFPE samples analysis with the DEPArray technology visit our FFPE dedicated page

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