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Ampli1™ LowPass Kit


Ampli
1™ LowPass Kit applications include the characterization and investigation of genomic heterogeneity and evolution in tumor cell populations such as individual circulating tumor cells (CTCs)

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Ampli1™ LowPass Kit is designed to generate multiplexed, sequencing-ready libraries in a streamlined single-day protocol, to detect chromosomal aneuploidies and copy number aberrations (CNAs) by low-pass whole genome sequencing.

Ampli1™ LowPass Kit is compatible with Ion Torrent systems (Ion PGM™ and Ion S5™) and NOW also compatible with Illumina® systems (MiSeq®, HiSeq® 1000/1500, 2000/2500, 3000/4000 and NovaSeq™).


 The Ampli1™ LowPass Kit works exclusively with Ampli1WGA products. The complete workflow takes advantage of the isolation of 100% pure cells with the DEPArray system and balanced whole genome amplification using the Ampli1™ WGA kit. APPLICATIONS_Oncology_CTC_Ampli1.png


Performance of this method has been extensively validated by analyzing the Copy Number profiles of single CTCs and WBCs from cancer patients (see figure below for representative data). 

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Low-pass Whole Genome Profile (chr 1-22) of single CTC (upper panel) and WBC (lower panel) isolated with DEPArray Technology from a patient with Lung Adenocarcinoma. Ploidy values are indicated in the y-axis; in the x-axis the alternation of different chromosomes are plotted with different colors. CNAs in the CTC are indicated in red and blue.


Ampli1™ LowPass Kit for Ion Torrent -  Overview:

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  • Two-step procedure that avoids the laborious fragmentation step and preparation of several enzyme reactions.
  • Up to 96 barcoded libraries suitable for both Ion Torrent NGS platforms: Ion PGM™ and Ion S5™.
  • Accurate characterization of CNA profiles of multiple single tumor cells per single run.
  • Fast results.

Ampli1™ LowPass Kit for Illumina -  Overview:

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  • Three-step procedure based on a novel, fragmentation-free and size selection-free method.
  • Single-tube prep method.
  • Up to 96 barcoded libraries suitable for Illumina MiSeq®, HiSeq® 1000/1500, 2000/2500, 3000/4000 and NovaSeq™ sequencing instruments.
  • Suitable for high-throughput liquid handling automation.

References:

Buson G et al, AACR 2016, “Scalable, rapid and affordable low-pass whole genome
sequencing method for single-cell copy-number profiling on LM-PCR based WGA products”

Ferrarini A et al, AACR 2016, “Precise copy-number profiling of single cells isolated from FFPE
tissues by low-pass whole-genome sequencing”

To download the posters please go to our Posters page.

To download Material Safety Data Sheets (MSDS), please click here.

The Ampli1™ WGA Kit and Ampli1™ LowPass Kits are for Research Use Only. Not for use in diagnostic procedures. Ampli1™ WGA Kits are protected by U.S. patent 6,673,541 and corresponding patent claims outside the United States.

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